An organism-wide atlas of hormonal signaling based on the mouse lemur single-cell transcriptome

Author:

Liu ShixuanORCID,Ezran CamilleORCID,Wang Michael F. Z.,Li Zhengda,Awayan KyleORCID, ,Agarwal Snigdha,Agrawal Aditi,Al-Moujahed Ahmad,Alam Alina,Albertelli Megan A.,Allegakoen Paul,Ambrosi Thomas,Antony Jane,Artandi Steven,Aujard Fabienne,Baghel Ankit,Bakerman Isaac,Bakken Trygve. E.,Baruni Jalal,Beachy Philip,Bilen Biter,Botvinnik Olga,Boyd Scott. D.,Burhan Deviana,Casey Kerriann M.,Chan Charles,Chang Charles. A.,Chang Stephen,Chen Ming,Clarke Michael F.,Crasta Sheela,Culver Rebecca,D’Addabbo Jessica,Darmanis Spyros,Dehghannasiri Roozbeh,Ding Song-Lin,Duffy Connor V.,Espinoza F. Hernán,Farup Jean,Frank Hannah K.,Fuller Margaret,Gillich Astrid,Godoy Elias,Gratzinger Dita,Guethlein Lisbeth A.,Hang Yan,Hasegawa Kazuteru,Hodge Rebecca D.,Hoover Malachia,Huang Franklin W.,Huang Kerwyn C.,Huynh Shelly,Isobe Taichi,Israel Carly,Jang SoRi,Jing Qiuyu,Jones Robert C.,Kang Jengmin,Karanewsky Caitlin J.,Karkanias Jim,Kebschull Justus,Kershner Aaron,Kim Lily,Kim Seung K.,Kirk E. Christopher,Koh Winston,Konermann Silvana,Kong William,Lautier Corinne,Lee Song Eun,Lein Ed S.,Lewis Rebecca,Li Peng,Lin Shengda,Liu Yin,Loeb Gabriel,Lu Wan-Jin,Lucot Katherine,Luo Liqun,Maynard Ashley,McGeever Aaron,Metzger Ross,Ming Jingsi,Montine Tom,de Morree Antoine,Morri Maurizio,Mrouj Karim,Mukherjee Shravani,Nabhan Ahmad,Nafees Saba,Neff Norma,Neuhöfer Patrick,Nguyen Patricia,Okamoto Jennifer,Olivieri Julia,Ouadah Youcef,Paine Honor,Parham Peter,Pendleton Jozeph L.,Penland Lolita,Perret Martine,Pisco Angela Oliveira,Qi Zhen,Quake Stephen R.,Radespiel Ute,Rando Thomas A.,Noëline Ravelonjanahary Hajanirina,Razafindrakoto Andriamahery,Salzman Julia,Schaum Nicholas,Schopler Robert,Scott Bronwyn,Shapiro Liza,Sin Hosu,Sinha Rahul,Sit Rene,Stanley Geoff,Stryer Lubert,Subramaniam Varun Ramanan,Swarup Aditi,Tan Michelle,Tan Weilun,Tarashansky Alexander,Taychameekiatchai Aris,Travaglini Kyle J.,Urtasun Andoni,Sivakamasundari ,Veerakumar Avin,Vemuri Venkata N. P.,Verdier Jean-Michel,Vollrath Douglas,Wang Bo,Wang Bruce,Wang Gefei,Webber James,Weinstein Hannah,Weissman Irving L.,Wiggenhorn Amanda L.,Williams Cathy V.,Wright Patricia,Wu Albert Y.,Wu Angela Ruohao,Wu Timothy Ting-Hsuan,Wyss-Coray Tony,Li BaoXiang,Yan Jia,Yang Can,Yang Jinxurong,Yoder Anne D.,Yu Brian,Yung Andrea R.,Zhang Yue,Zhao Jia,Zhao Zicheng,Long Jonathan Z.ORCID,De Vlaminck Iwijn,Wang ShengORCID,Epelbaum Jacques,Kuo Christin S.,Terrien Jérémy,Krasnow Mark A.ORCID,Ferrell James E.ORCID

Abstract

AbstractHormones mediate long-range cell communication and play vital roles in physiology, metabolism, and health. Traditionally, endocrinologists have focused on one hormone or organ system at a time. Yet, hormone signaling by its very nature connects cells of different organs and involves crosstalk of different hormones. Here, we leverage the organism-wide single cell transcriptional atlas of a non-human primate, the mouse lemur (Microcebus murinus), to systematically map source and target cells for 84 classes of hormones. This work uncovers previously-uncharacterized sites of hormone regulation, and shows that the hormonal signaling network is densely connected, decentralized, and rich in feedback loops. Evolutionary comparisons of hormonal genes and their expression patterns show that mouse lemur better models human hormonal signaling than mouse, at both the genomic and transcriptomic levels, and reveal primate-specific rewiring of hormone-producing/target cells. This work complements the scale and resolution of classical endocrine studies and sheds light on primate hormone regulation.

Funder

U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences

The Wall Center and Wu Tsai grants are internal Stanford grants

Publisher

Springer Science and Business Media LLC

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