A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes

Author:

Darnaud MarionORCID,De Vadder FilipeORCID,Bogeat Pascaline,Boucinha Lilia,Bulteau Anne-Laure,Bunescu AndreiORCID,Couturier CélineORCID,Delgado AnaORCID,Dugua Hélène,Elie Céline,Mathieu Alban,Novotná Tereza,Ouattara Djomangan Adama,Planel Séverine,Saliou Adrien,Šrůtková Dagmar,Yansouni Jennifer,Stecher Bärbel,Schwarzer MartinORCID,Leulier FrançoisORCID,Tamellini AndreaORCID

Abstract

AbstractMus musculus is the classic mammalian model for biomedical research. Despite global efforts to standardize breeding and experimental procedures, the undefined composition and interindividual diversity of the microbiota of laboratory mice remains a limitation. In an attempt to standardize the gut microbiome in preclinical mouse studies, here we report the development of a simplified mouse microbiota composed of 15 strains from 7 of the 20 most prevalent bacterial families representative of the fecal microbiota of C57BL/6J Specific (and Opportunistic) Pathogen-Free (SPF/SOPF) animals and the derivation of a standardized gnotobiotic mouse model called GM15. GM15 recapitulates extensively the functionalities found in the C57BL/6J SOPF microbiota metagenome, and GM15 animals are phenotypically similar to SOPF or SPF animals in two different facilities. They are also less sensitive to the deleterious effects of post-weaning malnutrition. In this work, we show that the GM15 model provides increased reproducibility and robustness of preclinical studies by limiting the confounding effect of fluctuation in microbiota composition, and offers opportunities for research focused on how the microbiota shapes host physiology in health and disease.

Funder

French Government as part of the Programme des Investissements d’Avenir

Czech Science Foundation and EMBO Installation grant

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

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