Magneto-optical diagnosis of symptomatic malaria in Papua New Guinea
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Published:2021-02-12
Issue:1
Volume:12
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Arndt L., Koleala T., Orbán Á., Ibam C., Lufele E.ORCID, Timinao L.ORCID, Lorry L., Butykai Á., Kaman P., Molnár A. P., Krohns S.ORCID, Nate E., Kucsera I., Orosz E., Moore B., Robinson L. J., Laman M., Kézsmárki I., Karl S.ORCID
Abstract
AbstractImproved methods for malaria diagnosis are urgently needed. Here, we evaluate a novel method named rotating-crystal magneto-optical detection (RMOD) in 956 suspected malaria patients in Papua New Guinea. RMOD tests can be conducted within minutes and at low cost. We systematically evaluate the capability of RMOD to detect infections by directly comparing it with expert light microscopy, rapid diagnostic tests and polymerase chain reaction on capillary blood samples. We show that compared to light microscopy, RMOD exhibits 82% sensitivity and 84% specificity to detect any malaria infection and 87% sensitivity and 88% specificity to detect Plasmodium vivax. This indicates that RMOD could be useful in P. vivax dominated elimination settings. Parasite density correlates well with the quantitative magneto-optical signal. Importantly, residual hemozoin present in malaria-negative patients is also detectable by RMOD, indicating its ability to detect previous infections. This could be exploited to reveal transmission hotspots in low-transmission settings.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Reference74 articles.
1. Sachs, J. & Malaney, P. The economic and social burden of malaria. Nature 415, 680–685 (2002). 2. Pagola, S., Stephens, P. W., Bohle, D. S., Kosar, A. D. & Madsen, S. K. The structure of malaria pigment beta-haematin. Nature 404, 307–310 (2000). 3. Feachem, R. G., Phillips, A. A., Targett, G. A. & Snow, R. W. Call to action: priorities for malaria elimination. Lancet 376, 1517–1521 (2010). 4. Hackett, S., Hamzah, J., Davis, T. M. & St Pierre, T. G. Magnetic susceptibility of iron in malaria-infected red blood cells. Biochim. Biophys. Acta 1792, 93–99 (2009). 5. Paul, F., Roath, S., Melville, D., Warhurst, D. C. & Osisanya, J. O. Separation of malaria-infected erythrocytes from whole blood: use of a selective high-gradient magnetic separation technique. Lancet 2, 70–71 (1981).
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