Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals

Author:

Yevtodiyenko Aleksey,Bazhin ArkadiyORCID,Khodakivskyi PavloORCID,Godinat Aurelien,Budin Ghyslain,Maric TamaraORCID,Pietramaggiori Giorgio,Scherer Sandra S.,Kunchulia Marina,Eppeldauer George,Polyakov Sergey V.,Francis Kevin P.,Bryan Jeffrey N.ORCID,Goun Elena A.

Abstract

AbstractBioluminescent imaging (BLI) is one of the most powerful and widely used preclinical imaging modalities. However, the current technology relies on the use of transgenic luciferase-expressing cells and animals and therefore can only be applied to a limited number of existing animal models of human disease. Here, we report the development of a “portable bioluminescent” (PBL) technology that overcomes most of the major limitations of traditional BLI. We demonstrate that the PBL method is capable of noninvasive measuring the activity of both extracellular (e.g., dipeptidyl peptidase 4) and intracellular (e.g., cytochrome P450) enzymes in vivo in non-luciferase-expressing mice. Moreover, we successfully utilize PBL technology in dogs and human cadaver, paving the way for the translation of functional BLI to the noninvasive quantification of biological processes in large animals. The PBL methodology can be easily adapted for the noninvasive monitoring of a plethora of diseases across multiple species.

Funder

Swiss National Science Foundation | National Center of Competence in Research Affective Sciences - Emotions in Individual Behaviour and Social Processes

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

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