Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing-Reference-Cited by-同舟云学术

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Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing

Published:2024-09-04 Issue:1 Volume:15 Page:
ISSN:2041-1723
Container-title:Nature Communications
language:en
Short-container-title:Nat Commun

Author:

Abe Kaitlyn M.^ORCID,Li Gan,He Qixiang^ORCID,Grant Timothy^ORCID,Lim Ci Ji^ORCID

Funder

U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences

Publisher

Springer Science and Business Media LLC

Link

https://www.nature.com/articles/s41467-024-52091-1.pdf

Reference74 articles.

1. Kühlbrandt, W. The resolution revolution. Science 343, 1443–1444 (2014).

2. Han, B.-G., Avila-Sakar, A., Remis, J. & Glaeser, R. M. Challenges in making ideal cryo-EM samples. Curr. Opin. Struct. Biol. 81, 102646 (2023).

3. Xu, Y. & Dang, S. Recent technical advances in sample preparation for single-particle cryo-EM. Front. Mol. Biosci. 9, 892459 (2022).

4. D’Imprima, E. et al. Protein denaturation at the air-water interface and how to prevent it. Elife 8, e42747 (2019).

5. Noble, A. J. et al. Reducing effects of particle adsorption to the air–water interface in cryo-EM. Nat. Methods 15, 793–795 (2018).

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