A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice-Reference-Cited by-同舟云学术

A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

Published:2019-09-03 Issue:1 Volume:10 Page:
ISSN:2041-1723
Container-title:Nature Communications
language:en
Short-container-title:Nat Commun

Author:

Young Joanna^ORCID,Dominicus Caia^ORCID,Wagener Jeanette,Butterworth Simon^ORCID,Ye Xingda^ORCID,Kelly Gavin^ORCID,Ordan Merav,Saunders Becky,Instrell Rachael,Howell Michael^ORCID,Stewart Aengus^ORCID,Treeck Moritz^ORCID

Funder

Francis Crick Institute

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

Link

http://www.nature.com/articles/s41467-019-11855-w.pdf

Reference44 articles.

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2. Wang, T., Wei, J. J., Sabatini, D. M. & Lander, E. S. Genetic screens in human cells using the CRISPR-Cas9 system. Science 343, 80–84 (2014).

3. Wang, T. et al. Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance. Nat. Commun. 9, 2475 (2018).

4. Blondel, C. J. et al. CRISPR/Cas9 screens reveal requirements for host cell sulfation and fucosylation in bacterial type III secretion system-mediated cytotoxicity. Cell Host Microbe 20, 226–237 (2016).

5. Pacheco, A. R. et al. CRISPR screen reveals that EHEC’s T3SS and Shiga toxin rely on shared host factors for infection. mBio 9, e01003–e01018 (2018).

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