Abstract
AbstractAccurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtubules during cell division. Branching microtubule nucleation relies on the hetero-octameric augmin complex, but lack of structure information about augmin has hindered understanding how it promotes branching. In this work, we combine cryo-electron microscopy, protein structural prediction, and visualization of fused bulky tags via negative stain electron microscopy to identify the location and orientation of each subunit within the augmin structure. Evolutionary analysis shows that augmin’s structure is highly conserved across eukaryotes, and that augmin contains a previously unidentified microtubule binding site. Thus, our findings provide insight into the mechanism of branching microtubule nucleation.
Funder
U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
Reference56 articles.
1. Salmon, E. D., Leslie, R. J., Saxton, W. M., Karow, M. L. & McIntosh, J. R. Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching. J. Cell Biol. 99, 2165–2174 (1984).
2. Mirny, L. A. & Needleman, D. J. Quantitative characterization of filament dynamics by single-molecule lifetime measurements. Methods Cell Biol. 95, 583–600 (2010).
3. Hoffmann, I. Centrosomes in mitotic spindle assembly and orientation. Curr. Opin. Struct. Biol. 66, 193–198 (2021).
4. Wu, J. & Akhmanova, A. Microtubule-organizing centers. Annu Rev. Cell Dev. Biol. 33, 51–75 (2017).
5. Meunier, S. & Vernos, I. Acentrosomal microtubule assembly in mitosis: The where, when, and how. Trends Cell Biol. 26, 80–87 (2016).
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