Abstract
AbstractSingle-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.
Funder
RCUK | Engineering and Physical Sciences Research Council
RCUK | STFC | Central Laser Facility, Science and Technology Facilities Council
EC | Erasmus+
RCUK | Biotechnology and Biological Sciences Research Council
Royal Society
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Cited by
31 articles.
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