N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity
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Published:2023-10-27
Issue:1
Volume:14
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Varland Sylvia, Silva Rui DuarteORCID, Kjosås IneORCID, Faustino AlexandraORCID, Bogaert Annelies, Billmann MaximilianORCID, Boukhatmi HadiORCID, Kellen Barbara, Costanzo MichaelORCID, Drazic Adrian, Osberg Camilla, Chan Katherine, Zhang XiangORCID, Tong Amy Hin Yan, Andreazza Simonetta, Lee Juliette J., Nedyalkova Lyudmila, Ušaj Matej, Whitworth Alexander J.ORCID, Andrews Brenda J.ORCID, Moffat Jason, Myers Chad L.ORCID, Gevaert KrisORCID, Boone CharlesORCID, Martinho Rui Gonçalo, Arnesen ThomasORCID
Abstract
AbstractMost eukaryotic proteins are N-terminally acetylated, but the functional impact on a global scale has remained obscure. Using genome-wide CRISPR knockout screens in human cells, we reveal a strong genetic dependency between a major N-terminal acetyltransferase and specific ubiquitin ligases. Biochemical analyses uncover that both the ubiquitin ligase complex UBR4-KCMF1 and the acetyltransferase NatC recognize proteins bearing an unacetylated N-terminal methionine followed by a hydrophobic residue. NatC KO-induced protein degradation and phenotypes are reversed by UBR knockdown, demonstrating the central cellular role of this interplay. We reveal that loss of Drosophila NatC is associated with male sterility, reduced longevity, and age-dependent loss of motility due to developmental muscle defects. Remarkably, muscle-specific overexpression of UbcE2M, one of the proteins targeted for NatC KO-mediated degradation, suppresses defects of NatC deletion. In conclusion, NatC-mediated N-terminal acetylation acts as a protective mechanism against protein degradation, which is relevant for increased longevity and motility.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
Reference134 articles.
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