In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Author:

Høgset Håkon,Horgan Conor C.ORCID,Armstrong James P. K.,Bergholt Mads S.,Torraca VincenzoORCID,Chen Qu,Keane Timothy J.,Bugeon Laurence,Dallman Margaret J.ORCID,Mostowy SergeORCID,Stevens Molly M.ORCID

Abstract

AbstractZebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos.

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

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