Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion
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Published:2020-04-24
Issue:1
Volume:11
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Horstmann Julia A., Lunelli MicheleORCID, Cazzola Hélène, Heidemann Johannes, Kühne Caroline, Steffen Pascal, Szefs Sandra, Rossi Claire, Lokareddy Ravi K., Wang Chu, Lemaire Laurine, Hughes Kelly T., Uetrecht CharlotteORCID, Schlüter Hartmut, Grassl Guntram A., Stradal Theresia E. B.ORCID, Rossez Yannick, Kolbe Michael, Erhardt MarcORCID
Abstract
AbstractThe long external filament of bacterial flagella is composed of several thousand copies of a single protein, flagellin. Here, we explore the role played by lysine methylation of flagellin in Salmonella, which requires the methylase FliB. We show that both flagellins of Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed lysine residues by FliB. A Salmonella Typhimurium mutant deficient in flagellin methylation is outcompeted for gut colonization in a gastroenteritis mouse model, and methylation of flagellin promotes bacterial invasion of epithelial cells in vitro. Lysine methylation increases the surface hydrophobicity of flagellin, and enhances flagella-dependent adhesion of Salmonella to phosphatidylcholine vesicles and epithelial cells. Therefore, posttranslational methylation of flagellin facilitates adhesion of Salmonella Typhimurium to hydrophobic host cell surfaces, and contributes to efficient gut colonization and host infection.
Funder
EC | European Regional Development Fund Helmholtz Association Leibniz grant SAW-2014-HPI-4
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
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