A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Link
http://www.nature.com/articles/s41467-021-22795-9.pdf
Reference38 articles.
1. Scott, M., Gunderson, C. W., Mateescu, E. M., Zhang, Z. & Hwa, T. Interdependence of cell growth and gene expression: origins and consequences. Science 330, 1099–1102 (2010).
2. Gyorgy, A. et al. Isocost lines describe the cellular economy of genetic circuits. Biophys. J. 109, 639–646 (2015).
3. Ceroni, F. et al. Burden-driven feedback control of gene expression. Nat. Methods 15, 387 (2018).
4. Ceroni, F., Algar, R., Stan, G. B. & Ellis, T. Quantifying cellular capacity identifies gene expression designs with reduced burden. Nat. Methods 12, 415 (2015).
5. Huliak, I., Sike, A., Zencir, S. & Boros, I. M. The objectivity of reporters: interference between physically unlinked promoters affects reporter gene expression in transient transfection experiments. DNA Cell Biol. 31, 1580–1584 (2012).
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