Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Author:

Bowers Robert M, ,Kyrpides Nikos C,Stepanauskas RamunasORCID,Harmon-Smith Miranda,Doud Devin,Reddy T B K,Schulz FrederikORCID,Jarett Jessica,Rivers Adam R,Eloe-Fadrosh Emiley A,Tringe Susannah GORCID,Ivanova Natalia N,Copeland Alex,Clum Alicia,Becraft Eric D,Malmstrom Rex R,Birren Bruce,Podar Mircea,Bork Peer,Weinstock George M,Garrity George M,Dodsworth Jeremy A,Yooseph Shibu,Sutton GrangerORCID,Glöckner Frank O,Gilbert Jack A,Nelson William C,Hallam Steven J,Jungbluth Sean PORCID,Ettema Thijs J G,Tighe Scott,Konstantinidis Konstantinos T,Liu Wen-Tso,Baker Brett J,Rattei Thomas,Eisen Jonathan A,Hedlund Brian,McMahon Katherine D,Fierer Noah,Knight RobORCID,Finn Rob,Cochrane Guy,Karsch-Mizrachi Ilene,Tyson Gene W,Rinke ChristianORCID,Lapidus AllaORCID,Meyer Folker,Yilmaz PelinORCID,Parks Donovan HORCID,Murat Eren AORCID,Schriml Lynn,Banfield Jillian F,Hugenholtz Philip,Woyke Tanja

Abstract

AbstractWe present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

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