Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis-Reference-Cited by-同舟云学术

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Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis

Published:2014-07-13 Issue:9 Volume:11 Page:966-970
ISSN:1548-7091
Container-title:Nature Methods
language:en
Short-container-title:Nat Methods

Author:

Lyozin George T,Bressloff Paul C,Kumar Amit,Kosaka Yasuhiro,Demarest Bradley L,Yost H Joseph,Kuehn Michael R,Brunelli Luca

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biochemistry,Biotechnology

Link

http://www.nature.com/articles/nmeth.3030.pdf

Reference38 articles.

1. Zhang, Y., Buchholz, F., Muyrers, J.P. & Stewart, A.F. A new logic for DNA engineering using recombination in Escherichia coli. Nat. Genet. 20, 123–128 (1998).

2. Murphy, K.C. Use of bacteriophage λ recombination functions to promote gene replacement in Escherichia coli. J. Bacteriol. 180, 2063–2071 (1998).

3. Yu, D. et al. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97, 5978–5983 (2000).

4. Skarnes, W.C. et al. A conditional knockout resource for the genome-wide study of mouse gene function. Nature 474, 337–342 (2011).

5. Sharan, S.K., Thomason, L.C., Kuznetsov, S.G. & Court, D.L. Recombineering: a homologous recombination-based method of genetic engineering. Nat. Protoc. 4, 206–223 (2009).

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