Author:
Ferreira J. R.,Caldeira J.,Sousa M.,Barbosa M. A.,Lamghari M.,Almeida-Porada G.,Gonçalves R. M.
Abstract
AbstractIntervertebral Disc (IVD) degeneration has been associated with a chronic inflammatory response, but knowledge on the contribution of distinct IVD cells, namely CD44, to the progression of IVD degeneration remains elusive. Here, bovine nucleus pulposus (NP) CD44 cells were sorted and compared by gene expression and proteomics with the negative counterpart. NP cells were then stimulated with IL-1b (10 ng/ml) and dynamics of CD44 gene and protein expression was analyzed upon pro-inflammatory treatment. The results emphasize that CD44 has a multidimensional functional role in IVD metabolism, ECM synthesis and production of neuropermissive factors. CD44 widespread expression in NP was partially associated with CD14 and CD45, resulting in the identification of distinct cell subsets. In conclusion, this study points out CD44 and CD44-based cell subsets as relevant targets in the modulation of the IVD pro-inflammatory/degenerative cascade.
Publisher
Springer Science and Business Media LLC
Reference84 articles.
1. Comer, C. & Conaghan, P. G. Tackling persistent low back pain in primary care. Practitioner. 253(1721), 32–43 (2009).
2. Risbud, M. & Shapiro, I. M. Role of cytokines in intervertebral disc degeneration: pain and disc content. Nat. Rev. Rheumatol. 10(1), 44–56 (2014).
3. Le Maitre, C., Hoyland, J. & Freemont, A. Catabolic cytokine expression in degenerate and herniated human intervertebral discs: IL-1beta and TNFalpha expression profile. Arthritis Res. Ther. 9(4), R77 (2007).
4. Van den Akker, G. G. et al. Novel immortal human cell lines reveal subpopulations in the nucleus pulposus. Arthritis Res. Ther. 16, 1–16 (2014).
5. Chelberg, M. K., Banks, G. M., Geiger, D. F. & Oegema, T. R. Jr. Identification of heterogeneous cell populations in normal human intervertebral disc. J. Anat. 186(Pt 1), 43 (1995).
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