Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT-Reference-Cited by-同舟云学术

Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT

Published:2017-11-27 Issue:1 Volume:7 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Shen Chuanlai^ORCID,Xu Tao,Wu You,Li Xiaoe,Xia Lingzhi,Wang Wei,Shahzad Khawar Ali,Zhang Lei,Wan Xin,Qiu Jie

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

http://www.nature.com/articles/s41598-017-16549-1.pdf

Reference26 articles.

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2. Greten, T. F. et al. Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19-specific CD8 + T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients. Proceedings of the National Academy of Sciences 95, 7568–7573 (1998).

3. He, X.-S. et al. Direct functional analysis of epitope-specific CD8 + T cells in peripheral blood. Viral immunology 14, 59–69, https://doi.org/10.1089/08828240151061400 (2001).

4. Appay, V. & Rowland-Jones, S. L. The assessment of antigen-specific CD8 + T cells through the combination of MHC class I tetramer and intracellular staining. Journal of immunological methods 268, 9–19 (2002).

5. Betts, M. R. et al. Sensitive and viable identification of antigen-specific CD8 + T cells by a flow cytometric assay for degranulation. Journal of immunological methods 281, 65–78, https://doi.org/10.1016/S0022-1759(03)00265-5 (2003).

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