A novel noninvasive approach for evaluating work of breathing indices in a developmental rat model using respiratory inductance plethysmography
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Published:2020-11-26
Issue:1
Volume:10
Page:
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ISSN:2045-2322
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Container-title:Scientific Reports
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language:en
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Short-container-title:Sci Rep
Author:
Zhu Yan,Aghai Zubair H.,Betal Suhita Gayen Nee,Favara Michael,Fong Gina,Rahman Tariq,Shaffer Thomas H.
Abstract
AbstractPulmonary function testing (PFT) is an important component for evaluating the outcome of experimental rodent models of respiratory diseases. Respiratory inductance plethysmography (RIP) provides a noninvasive method of PFT requiring minimal cooperation. RIP measures work of breathing (WOB) indices including phase angle (Ф), percent rib cage (RC %), breaths per minute (BPM), and labored breathing index (LBI) on an iPad. The aim of this study was to evaluate the utility of a recently developed research instrument, pneuRIP, for evaluation of WOB indices in a developmental rat model. Sprague Dawley rats (2 months old) were commercially acquired and anaesthetised with isoflurane. The pneuRIP system uses two elastic bands: one band (RC) placed around the rib cage under the upper armpit and another band (AB) around the abdomen. The typical thoracoabdominal motion (TAM) plot showed the abdomen and rib cage motion in synchrony. The plots of phase angle and LBI as a function of data point number showed that values were within the range. The distribution for phase angle and LBI was within a narrow range. pneuRIP testing provided instantaneous PFT results. This study demonstrated the utility of RIP as a rapid, noninvasive approach for evaluating treatment interventions in the rodent model.
Funder
The Nemours Foundation
University of Delaware, Center for Advanced Technology (CAT) Program
Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
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