Author:
Oliveira Jairo Pinto,Prado Adilson Ribeiro,Keijok Wanderson Juvencio,Antunes Paulo Wagnner Pereira,Yapuchura Enrique Ronald,Guimarães Marco Cesar Cunegundes
Abstract
Abstract
Antibody-coated nanoparticles have recently attracted considerable attention, with the focus falling on diagnostics. Nevertheless, controlled antibody bioconjugation remains a challenge. Here, we present two strategies of bioconjugation with the aim of evaluating the best approach for the coupling of antibodies on the surface of nanomaterials in an oriented way. We employed electrostatic interaction (physical adsorption) and covalent conjugation in the orientation of antibodies on the metallic surface as coupling methods, and their influence on the detection of 17β-estradiol was addressed with localized surface plasmon resonance. The understanding of these mechanisms is fundamental for the development of reproducible inorganic bioconjugates with oriented surface as well sensibility of immunoassays.
Publisher
Springer Science and Business Media LLC
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