Highly efficient DNA-free gene disruption in the agricultural pest Ceratitis capitata by CRISPR-Cas9 ribonucleoprotein complexes

Author:

Meccariello Angela,Monti Simona Maria,Romanelli AlessandraORCID,Colonna Rita,Primo Pasquale,Inghilterra Maria Grazia,Del Corsano Giuseppe,Ramaglia Antonio,Iazzetti Giovanni,Chiarore Antonia,Patti Francesco,Heinze Svenia D.,Salvemini Marco,Lindsay Helen,Chiavacci Elena,Burger Alexa,Robinson Mark D.ORCID,Mosimann ChristianORCID,Bopp DanielORCID,Saccone GiuseppeORCID

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Reference67 articles.

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2. Dyck, V. A., Hendrics., J., & Robinson, A. S. Sterile Insect Technique. 1–787 (Springer Netherlands, 2005).

3. Morrison, N. I. et al. Genetic improvements to the sterile insect technique for agricultural pests. Asia Pacific Journal of Molecular Biology and Biotechnology 18, 275–295 (2010).

4. Loukeris, T. G., Livadaras, I., Arca, B., Zabalou, S. & Savakis, C. Gene-transfer into the medfly, Ceratitis capitata, with a Drosophila hydei transposable element. Science 270, 2002–2005 (1995).

5. Zwiebel, L. J. et al. The white gene of Ceratitis capitata - a phenotypic marker for germline transformation. Science 270, 2005–2008 (1995).

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