Author:
Kumar Dinesh,Ladaniya M. S.,Gurjar Manju,Kumar Sunil
Abstract
AbstractCitrus fruits are famous for nutritional value and studies are there for extraction of secondary metabolites from citrus waste. An attempt was made to quantify antioxidants, flavonoids and phenols from dropped fruits of 8–24 mm size, to find the impact of freeze and hot-air oven drying techniques on extraction. Flavonoids (hesperidin, narirutin/isonaringin, diosmin and didymin/neoponcirin) were quantified through high performance liquid chromatography (HPLC) and total phenols (TPC) were estimated by Folin-Ciocalteu method. Antioxidant capacity was adjudged by azino-bis [3-ethylbenzthiazoline-6-sulfonic acid] (ABTS), 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) and Ferric Reducing Antioxidant Power (FRAP). Freeze dried fruits of 10 mm and 12 mm retained maximum hesperidin content (22.383% and 21.560%) in comparison to hot-air oven counterparts (18.377% and 15.090%). Narirutin/isonaringin (1.343% and 1.191%), diosmin (5.293% and 3.234%) and didymin/neoponcirin (1.187% and 1.113%) content were found higher in 8 mm and 10 mm freeze dried fruits. The antioxidant capacity (7.548–11.643 mmol L−1 Trolox, 8.164–14.710 mmol L−1 Trolox, 4.008–5.863 mmol L−1 Trolox by ABTS, DPPH and FRAP assays) and TPC were found higher in freeze dried samples. Significant correlation was found between antioxidant capacity, TPC and flavonoids at p < 0.01. Freeze drying technique can be adopted for retaining and quality extraction of bioactive compounds from immature dropped fruits for further use in nutraceutical industries.
Publisher
Springer Science and Business Media LLC
Reference49 articles.
1. Leporini, M., Tundis, R., Sicari, V. & Loizzo, M. R. Citrus species: Modern functional food and nutraceutical-based product ingredient. Ital. J. Food Sci. 33(2), 63–107. https://doi.org/10.15586/ijfs.v33i2.2009 (2021).
2. Kim, J. H. & Kim, M. Y. Phytochemical and antioxidant characterization of thinned immature Citrus unshiu fruits. Int. J. Pharm. Pharm. Sci. 9(12), 293–297. https://doi.org/10.22159/ijpps.2017v9i12.22971 (2017).
3. Favela-Hernández, J. M. J., González-Santiago, O., Ramírez-Cabrera, M. A., Esquivel-Ferriño, P. C. & Camacho-Corona, M. D. R. Chemistry and pharmacology of Citrus sinensis. Molecules 21, 247. https://doi.org/10.3390/molecules21020247 (2016).
4. Kumar, D., Ladaniya, M. S., Gurjar, M., Kumar, S. & Mendke, S. Quantification of Flavonoids, phenols and antioxidant potential from dropped Citrus reticulata Blanco fruits influenced by drying techniques. Molecules 26(4159), 2–13. https://doi.org/10.3390/molecules26144159 (2021).
5. Al-Juhaimi, F. Y. & Ghafoor, K. Bioactive compounds, antioxidant and physic-chemical properties of juice from lemon, mandarin and orange fruits cultivated in Saudi Arabia. Pak. J. Bot. 45(4), 1193–1196 (2013).