Efficient strategy for introducing large and multiple changes in plasmid DNA
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-018-20169-8.pdf
Reference34 articles.
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2. Rushworth, C. A., Guy, J. L. & Turner, A. J. Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis. FEBS J 275, 6033–6042, https://doi.org/10.1111/j.1742-4658.2008.06733.x (2008).
3. Hsieh, P. C. & Vaisvila, R. Protein engineering: single or multiple site-directed mutagenesis. Methods Mol Biol 978, 173–186, https://doi.org/10.1007/978-1-62703-293-3_13 (2013).
4. Liu, H. & Naismith, J. H. An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol. BMC Biotechnol 8, 91, https://doi.org/10.1186/1472-6750-8-91 (2008).
5. Pingoud, A. & Jeltsch, A. Structure and function of type II restriction endonucleases. Nucleic Acids Res 29, 3705–3727 (2001).
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