Author:
Santana Alexandre Chagas,Andraus Wellington,Zimelewicz Oberman Dan,Rabelo Nícollas Nunes,Silva Filipe Miranda Oliveira,Dellê Humberto,Pepineli Rafael,de Moraes Edvaldo Leal,Scavone Cristoforo,de Sá Lima Larissa,Degaspari Sabrina,Brasil Sérgio,Solla Davi Jorge Fontoura,Ruiz Liliane Moreira,de Oliveira-Braga Karina Andrighetti,Nepomuceno Natalia Aparecida,Pêgo-Fernandes Paulo Manuel,Tullius Stefan Gunther,Figueiredo Eberval Gadelha
Abstract
AbstractLiver transplantation has come a long way and is now regarded as the gold standard treatment for end-stage liver failure. The great majority of livers utilized in transplantation come from brain-dead donors. A broad inflammatory response characterizes BD, resulting in multiorgan damage. This process is primarily mediated by cytokines, which increase the immunogenicity of the graft. In male Lewis rats, we evaluated the immune response in a BD liver donor and compared it to that of a control group. We studied two groups: Control and BD (rats subjected to BD by increasing intracranial pressure). After the induction of BD, there was an intense rise in blood pressure followed by a fall. There were no significant differences observed between the groups. Blood tissue and hepatic tissue analyzes showed an increase in plasma concentrations of liver enzymes (AST, ALT, LDH and ALP), in addition to pro-inflammatory cytokines and macrophages in liver tissue in animals submitted to BD. The current study found that BD is a multifaceted process that elicits both a systemic immune response and a local inflammatory response in liver tissue. Our findings strongly suggested that the immunogenicity of plasma and liver increased with time following BD.
Publisher
Springer Science and Business Media LLC