Author:
Oh Mijung,Ha Dae-In,Son Chaeyeon,Kang Jeong Gu,Hwang Heeyoun,Moon Su Bin,Kim Minjeong,Nam Jihae,Kim Jung Soo,Song Sang Yong,Kim Yong-Sam,Park Sangwoo,Yoo Jong Shin,Ko Jeong-Heon,Park Kyoungsook
Abstract
AbstractSialic acid (SA) is present in glycoconjugates and important in cell–cell recognition, cell adhesion, and cell growth and as a receptor. Among the four mammalian sialidases, cytosolic NEU2 has a pivotal role in muscle and neuronal differentiation in vitro. However, its biological functions in vivo remain unclear due to its very low expression in humans. However, the presence of cytoplasmic glycoproteins, gangliosides, and lectins involved in cellular metabolism and glycan recognition has suggested the functional importance of cytosolic Neu2 sialidases. We generated a Neu2 knockout mouse model via CRISPR/Cas9-mediated genome engineering and analyzed the offspring littermates at different ages to investigate the in vivo function of cytosolic Neu2 sialidase. Surprisingly, knocking out the Neu2 gene in vivo abrogated overall lipid metabolism, impairing motor function and leading to diabetes. Consistent with these results, Neu2 knockout led to alterations in sialylated glycoproteins involved in lipid metabolism and muscle function, as shown by glycoproteomics analysis.
Funder
Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education
R&D Convergence Program of the NST of the Republic of Korea
Ministry of Science and ICT, Korea
Publisher
Springer Science and Business Media LLC
Cited by
5 articles.
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