Abstract
Abstract
The study describes a novel and environment friendly route of biosynthesis of nanohydroxyapatite (nHAP). Bacillus licheniformis mediated synthesis of nHAP has been carried out with different phosphate concentrations (2%, 5%, 10% and 20% w/v) of potassium dihydrogen orthophosphate monobasic (K2HPO4). The synthesis is supported by a two-step mechanism – (i) solubilization of P by organic acids extracellularly secreted by the bacterial strain and (ii) gelation of P and Ca. The nHAP particles were characterized using electron microscopy and XRD analysis. Powdered crystalline particles with a size range of 30 ± 5 nm were obtained with shape and size dependent on phosphate concentrations. The particles showed no adverse effect on plant growth-promoting bacteria. Evaluation of nHAP prepared by this route with 2% P source provides scope for a wide range of applications, especially as a nano-fertilizer.
Funder
Department of Biotechnology, Ministry of Science and Technology
Publisher
Springer Science and Business Media LLC
Reference59 articles.
1. Dey, P., Santhi, R., Maragatham, S. & Sellamuthu, K. Status of phosphorus and potassium in the Indian soils vis-à-vis world soils. Indian Journal of Fertilisers 13, 44–59 (2017).
2. Annual Report 2017–2018. 208 (Ministry of Agriculture & Farmers Welfare, Delhi, India, 2017).
3. Fageria, N. K. The Use of Nutrients in Crop Plants. (CPC Press, 2009).
4. Liu, R. & Lal, R. Synthetic apatite nanoparticles as a phosphorus fertilizer for soybean (Glycine max). Sci Rep 4, 5686,
https://doi.org/10.1038/srep05686
(2014).
5. Nikolay, V. et al. Solubilization of animal bonechar by a filamentous fungus employed in solid state fermentation. Ecological engineering 58, 165–169 (2013).
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