Author:
Jiang Lingling,Zhang Gang,Wang Pu,Niu Xiaoxia,Liu Qiang,Zhang Sinong,Gao Weifeng,Li Yong
Abstract
AbstractBovine viral diarrhea virus (BVDV) is considered to be the most common agent of severe diarrhea in cattle worldwide, causing fever, diarrhea, ulcers, and abortion. Bovine herpesvirus 1 (BoHV-1) is also a major bovine respiratory disease agent that spreads worldwide and causes extensive damage to the livestock industry. Recombinase polymerase amplification (RPA) is a novel nucleic acid amplification method with the advantages of high efficiency, rapidity and sensitivity, which has been widely used in the diagnosis of infectious diseases. A dual RPA assay was developed for the simultaneous detection of BVDV and BoHV-1. The assay was completed at a constant temperature of 37 °C for 30 min. It was highly sensitive and had no cross-reactivity with other common bovine viruses. The detection rate of BVDV RPA in clinical samples (36.67%) was higher than that of PCR (33.33%), the detection rate of BoHV-1 RPA and PCR were equal. Therefore, the established dual RPA assay for BVDV and BoHV-1 could be a potential candidate for use as an immediate diagnostic.
Funder
Ningxia Natural Science Foundation
The National Natural Science Foundation of China
The Key Research, Development Program of Ningxia Hui Autonomous Region
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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1. Severe adverse impact of bovine viral diarrhea on cattle production: A comprehensive approach to control;Zbornik radova 26. medunarodni kongres Mediteranske federacije za zdravlje i produkciju preživara - FeMeSPRum - zbornik radova;2024