Author:
Russi Romina Cecilia,Del Balzo Diego,Luján Agustín,Reidel Ivana Gabriela,García María Inés,Veaute Carolina,Damiani María Teresa
Abstract
AbstractThe control of the worldwide spread of sexually transmitted Chlamydia trachomatis (Ct) infection urgently demands the development of a preventive vaccine. In this work, we designed a vaccine based on a fragment of polymorphic protein D (FPmpD) that proved to be immunogenic enough to generate a robust systemic and mucosal IgG humoral immune response in two strains of mice. We used a heterologous prime-boost strategy, including simultaneous systemic and mucosal administration routes. The high titers of anti-PmpD antibodies elicited by this immunization scheme did not affect murine fertility. We tested the vaccine in a mouse model of Ct intravaginal infection. Anti-PmpD antibodies displayed potent neutralizing activity in vitro and protective effects in uterine tissues in vivo. Notably, the humoral immune response of PmpD-vaccinated mice was faster and stronger than the primary immune response of non-vaccinated mice when exposed to Ct. FPmpD-based vaccine effectively reduced Ct shedding into cervicovaginal fluids, bacterial burden at the genitourinary tract, and overall infectivity. Hence, the FPmpD-based vaccine might constitute an efficient tool to protect against Ct intravaginal infection and decrease the infection spreading.
Funder
Universidad Nacional del Litoral
Ministerio de Ciencia, Tecnología e Innovación Productiva de la Provincia de Santa Fe
Fondo para la Investigación Científica y Tecnológica
Secretaría de Investigación, Internacionales y Posgrado, Universidad Nacional de Cuyo
Publisher
Springer Science and Business Media LLC
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