The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)-Reference-Cited by-同舟云学术

The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)

Published:2024-08-07 Issue:1 Volume:14 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Sybilska Ewa^ORCID,Collin Anna^ORCID,Sadat Haddadi Bahareh,Mur Luis A. J.^ORCID,Beckmann Manfred^ORCID,Guo Wenbin^ORCID,Simpson Craig G.^ORCID,Daszkowska-Golec Agata^ORCID

Abstract

AbstractTo decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

Funder

Narodowe Centrum Nauki

Publisher

Springer Science and Business Media LLC

Link

https://www.nature.com/articles/s41598-024-69373-9.pdf

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