Author:
Chiu Yi-Ling,Chang Ching-Fong,Shikina Shinya
Abstract
AbstractIn vitro gonad culture systems have proven useful to investigate intrinsic mechanisms of sexual reproduction in animals. Here we describe development of an in vitro culture method for coral ovaries. Mesenterial tissues containing both ovaries and mesenterial filaments were microscopically isolated from the scleractinian coral, Fimbriaphyllia ancora, and culture conditions were optimized. M199 diluted 10× (10% M199, pH 8.1) and supplemented with 25 mM HEPES and the antibiotics, ampicillin, penicillin and streptomycin, supported oocyte survival and maintained the structural integrity of ovaries during short-term culture (~ 6 days). Addition of a commercial antibiotic–antimycotic solution (Anti–Anti) and fetal bovine serum adversely affected ovary maintenance and caused tissue disintegration. Characterization of cultured ovaries showed that there is no difference in cell proliferation of ovarian somatic cells between culture Days 1 and 6. Moreover, the presence of oogonia and expression of a major yolk protein, vitellogenin, were confirmed in ovaries cultured for 6 days. This system will be useful for studying effects of a wide range of substances on coral oogenesis.
Publisher
Springer Science and Business Media LLC
Reference69 articles.
1. Harrison, P. L. & Wallace, C. C. Reproduction, dispersal and recruitment of scleractinian corals. In Ecosystems of the World 25, Coral Reefs (eds Dubinsky, Z. & Stambler, N.) 133–207 (Elsevier, 1990).
2. Richmond, R. H. & Hunter, C. L. Reproduction and recruitment of corals: Comparisons among the Caribbean, the tropical Pacific, and the Red Sea. Mar. Ecol. Prog. Ser. 60, 185–203 (1990).
3. Baird, A. H., Guest, J. R. & Willis, B. L. Systematic and biogeographical patterns in the reproductive biology of scleractinian corals. Annu. Rev. Ecol. Evol. Syst. 40, 551–571 (2009).
4. Harrison, P. L. Sexual reproduction in scleractinian corals. In Coral Reefs: An Ecosystem in Transition (eds Dubinsky, Z. & Stambler, N.) 59–85 (Springer, 2011).
5. Pechenik, J. A. Biology of the Invertebrates 6th edn. (McGraw-Hill, Higher Education, 2010).
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