Author:
Capovilla Giovanna,Castro Kurt G.,Collani Silvio,Kearney Sean M.,Kehoe David M.,Chisholm Sallie W.
Abstract
AbstractChitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.
Publisher
Springer Science and Business Media LLC