Human dental pulp stem cells derived extracellular matrix promotes mineralization via Hippo and Wnt pathways-Reference-Cited by-同舟云学术

Human dental pulp stem cells derived extracellular matrix promotes mineralization via Hippo and Wnt pathways

Published:2024-03-21 Issue:1 Volume:14 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Kornsuthisopon Chatvadee,Nowwarote Nunthawan,Chansaenroj Ajjima,Photichailert Suphalak,Rochanavibhata Sunisa,Klincumhom Nuttha,Petit Stephane,Dingli Florent,Loew Damarys,Fournier Benjamin P. J.,Osathanon Thanaphum

Abstract

AbstractExtracellular matrix (ECM) is an intricate structure providing the microenvironment niche that influences stem cell differentiation. This study aimed to investigate the efficacy of decellularized ECM derived from human dental pulp stem cells (dECM_DPSCs) and gingival-derived mesenchymal stem cells (dECM_GSCs) as an inductive scaffold for osteogenic differentiation of GSCs. The proteomic analysis demonstrated that common and signature matrisome proteins from dECM_DPSCs and dECM_GSCs were related to osteogenesis/osteogenic differentiation. RNA sequencing data from GSCs reseeded on dECM_DPSCs revealed that dECM_DPSCs upregulated genes related to the Hippo and Wnt signaling pathways in GSCs. In the inhibitor experiments, results revealed that dECM_DPSCs superiorly promoted GSCs osteogenic differentiation, mainly mediated through Hippo and Wnt signaling. The present study emphasizes the promising translational application of dECM_DPSCs as a bio-scaffold rich in favorable regenerative microenvironment for tissue engineering.

Funder

Thailand Science Research and Innovation Fund Chulalongkorn University

Fondation des Gueules Cassées

INSERM/APHP Interface contract

Publisher

Springer Science and Business Media LLC

Link

https://www.nature.com/articles/s41598-024-56845-1.pdf

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