Optimization of ultrasonic pretreatment and analysis of chlorogenic acid in potato leaves-Reference-Cited by-同舟云学术

Optimization of ultrasonic pretreatment and analysis of chlorogenic acid in potato leaves

Published:2024-05-09 Issue:1 Volume:14 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Wang Xin,Gong Xianyun,Lin Binbin

Abstract

AbstractChlorogenic acid (CA) is an effective ingredient that can strengthen immunity during following the COVID-19 era. The current cost of CA is high owing to its complex purification process and low yield (approximately 2%). In this study, a one-step path orthogonal experiment was designed based on the results from Gauss calculation, which consisted of acidity, coordination, and hydrolysis in molecules. The optimized extraction conditions were 60 ℃, 60 min, 1:20 liquid ratio, and 40% ethanol in a nitrogen atmosphere controlled using a device of our own design, which led to CA yields of up to 6.35% from potato leaves. The purified CA was analyzed using high-performance liquid chromatography, thin-layer chromatography, ultraviolet–visible spectroscopy, and molecular fluorescence. This accurate and reproducible method can not only be used to obtain high yields of CA but can also be used for the quality control of active plant products and their isomers.

Publisher

Springer Science and Business Media LLC

Link

https://www.nature.com/articles/s41598-024-61139-7.pdf

Reference22 articles.

1. Gao, X. et al. Overview of research on medium and high frequency single drug of traditional Chinese medicine compound against novel coronavirus pneumonia (COVID-19). Foreign Med. 4, 283–289 (2020).

2. Liu, W., Gong, P. & Gu, J. Discussion on the treatment of novel coronavirus pneumonia (COVID-19) with antipyretic, anti-inflammatory and immunomodulatory Chinese medicine. Tradit. Chin. Med. 8, 2081–2088 (2020).

3. Zhang, B. et al. A monoclonal antibody-based enzyme-linked immunosorbent assay for the determination of chlorogenic acid in honeysuckle. J. Pharm. Biomed. Anal. 148, 1–5 (2018).

4. Zhu, W. et al. Research progress on functional components and biological activities of honeysuckle. Food Ind. Technol. 11, 26 (2020).

5. Li, H., Zhou, Z. & Chen, Y. Study on the separation process of chlorogenic acid and total flavonoids from Lonicera japonica using macroporous resin. Chem. World 11, 760–766 (2020).