Author:
Bukhari Tullu,Aimanianda Vishukumar,Bischoff Emmanuel,Brito-Fravallo Emma,Eiglmeier Karin,Riehle Michelle M.,Vernick Kenneth D.,Mitri Christian
Abstract
AbstractEntomopathogenic fungi have been explored as a potential biopesticide to counteract the insecticide resistance issue in mosquitoes. However, little is known about the possibility that genetic resistance to fungal biopesticides could evolve in mosquito populations. Here, we detected an important genetic component underlying Anopheles coluzzii survival after exposure to the entomopathogenic fungus Metarhizium anisopliae. A familiality study detected variation for survival among wild mosquito isofemale pedigrees, and genetic mapping identified two loci that significantly influence mosquito survival after fungus exposure. One locus overlaps with a previously reported locus for Anopheles susceptibility to the human malaria parasite Plasmodium falciparum. Candidate gene studies revealed that two LRR proteins encoded by APL1C and LRIM1 genes in this newly mapped locus are required for protection of female A. coluzzii from M. anisopliae, as is the complement-like factor Tep1. These results indicate that natural Anopheles populations already segregate frequent genetic variation for differential mosquito survival after fungal challenge and suggest a similarity in Anopheles protective responses against fungus and Plasmodium. However, this immune similarity raises the possibility that fungus-resistant mosquitoes could also display enhanced resistance to Plasmodium, suggesting an advantage of selecting for fungus resistance in vector populations to promote naturally diminished malaria vector competence.
Publisher
Springer Science and Business Media LLC
Reference49 articles.
1. Ranson, H. et al. Pyrethroid resistance in African anopheline mosquitoes: what are the implications for malaria control?. Trends Parasitol. 27, 91–98. https://doi.org/10.1016/j.pt.2010.08.004 (2011).
2. World Health Organization. World Malaria Report 2019, (2019).
3. Gari, T. & Lindtjorn, B. Reshaping the vector control strategy for malaria elimination in Ethiopia in the context of current evidence and new tools: opportunities and challenges. Malar. J. 17, 454. https://doi.org/10.1186/s12936-018-2607-8 (2018).
4. Riveron, J. M. et al. Insecticide resistance in malaria vectors: An update at a global scale 149–175 (IntechOpen, New York, 2018).
5. Lynch, P. A., Grimm, U., Thomas, M. B. & Read, A. F. Prospective malaria control using entomopathogenic fungi: Comparative evaluation of impact on transmission and selection for resistance. Malar. J. 11, 383. https://doi.org/10.1186/1475-2875-11-383 (2012).
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