Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum
Author:
Funder
Naito Foundation
Japan Society for the Promotion of Science
Asada Science Foundation
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-018-28896-8.pdf
Reference35 articles.
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2. Gil, L. et al. Current status of freeze-drying technology to preserve domestic animals sperm. Reproduction in domestic animals = Zuchthygiene 49(Suppl 4), 72–81, https://doi.org/10.1111/rda.12396 (2014).
3. Wakayama, T. & Yanagimachi, R. Development of normal mice from oocytes injected with freeze-dried spermatozoa. Nature biotechnology 16, 639–641 (1998).
4. Hirabayashi, M., Kato, M., Ito, J. & Hochi, S. Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads. Zygote (Cambridge, England) 13, 79–85 (2005).
5. Liu, J. L. et al. Freeze-dried sperm fertilization leads to full-term development in rabbits. Biology of reproduction 70, 1776–1781, https://doi.org/10.1095/biolreprod.103.025957 (2004).
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