Author:
Martínez-Santos Eduardo,Cruz-Cruz Carlos Alberto,Spinoso-Castillo José Luis,Bello-Bello Jericó Jabín
Abstract
AbstractDrought-induced water stress affects the productivity of the Vanilla planifolia Jacks. ex Andrews crop. In vitro culture technique is an effective tool for the study of water stress tolerance mechanisms. This study aimed to evaluate the morphological, physiological and biochemical response of V. planifolia under in vitro water stress conditions induced with polyethylene glycol (PEG). In vitro regenerated shoots of 2 cm in length were subjected to different concentrations of PEG 6000 (0, 1, 2 and 3% w/v) using Murashige and Skoog semi-solid culture medium. At 60 days of culture, different growth variables, dry matter (DM) content, chlorophyll (Chl), soluble proteins (SP), proline (Pro), glycine betaine (GB), stomatal index (SI) and open stomata (%) were evaluated. Results showed a reduction in growth, Chl content, SP, SI and open stomata (%) with increasing PEG concentration, whereas DM, Pro and GB contents rose with increasing PEG concentration. In conclusion, PEG-induced osmotic stress allowed describing physiological and biochemical mechanisms of response to water stress. Furthermore, the determination of compatible Pro and GB osmolytes can be used as biochemical markers in future breeding programs for the early selection of water stress tolerant genotypes.
Publisher
Springer Science and Business Media LLC
Reference65 articles.
1. Arya, S. S., Rookes, J. E., Cahill, D. M. & Lenka, S. K. Vanillin: A review on the therapeutic prospects of a popular flavouring molecule. Adv. Tradit. Med. https://doi.org/10.1007/s13596-020-00531-w (2021).
2. Castro-Bobadilla, G., Martínez, A. J., Martínez, M. L. & y García-Franco, J. G. Aplicación de riego localizado para aumentar la retención de frutos de Vanilla planifolia en el Totonacapan, Veracruz, México. Agrociencia 45(3), 281–291 (2011).
3. Li, J., Demesyeux, L., Brym, M. & Chambers, A. H. Development of species-specific molecular markers in Vanilla for seedling selection of hybrids. Mol. Biol. Rep. 47(3), 1905–1920. https://doi.org/10.1007/s11033-020-05287-9 (2020).
4. Monterroso-Rivas, A. I. & y Gómez-Díaz, J. D. Impacto del cambio climático en la evapotranspiración potencial y periodo de crecimiento en México. Terra LatAm. 39, 1–19. https://doi.org/10.28940/terra.v39i0.774 (2021).
5. Vinocur, B. & Altman, A. Recent advances in engineering plant tolerance to abiotic stress: Achievements and limitations. Curr. Opin. Biotechnol. 16(2), 123–132. https://doi.org/10.1016/j.copbio.2005.02.001 (2005).
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