Author:
Minami Shohei,Matsumoto Naomi,Omori Hiroko,Nakamura Yutaka,Tamiya Shigeyuki,Nouda Ryotaro,Nurdin Jeffery A.,Yamasaki Moeko,Kotaki Tomohiro,Kanai Yuta,Okamoto Toru,Tachibana Taro,Ushijima Hiroshi,Kobayashi Takeshi,Sato Shintaro
Abstract
AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe acute respiratory symptoms in humans. Controlling the coronavirus disease pandemic is a worldwide priority. The number of SARS-CoV-2 studies has dramatically increased, and the requirement for analytical tools is higher than ever. Here, we propose monolayered-intestinal epithelial cells (IECs) derived from human induced pluripotent stem cells (iPSCs) instead of three-dimensional cultured intestinal organoids as a suitable tool to study SARS-CoV-2 infection. Differentiated IEC monolayers express high levels of angiotensin-converting enzyme 2 and transmembrane protease serine 2 (TMPRSS2), host factors essential for SARS-CoV-2 infection. SARS-CoV-2 efficiently grows in IEC monolayers. Using this propagation system, we confirm that TMPRSS2 inhibition blocked SARS-CoV-2 infection in IECs. Hence, our iPSC-derived IEC monolayers are suitable for SARS-CoV-2 research under physiologically relevant conditions.
Funder
Japan Agency for Medical Research and Development
Publisher
Springer Science and Business Media LLC
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