Author:
Tian Mengyue,Li Nan,Liu Ruonan,Li Ke,Du Jinliang,Zou Dongmin,Ma Yuzhong
Abstract
AbstractLaminitis is one of the most important and intractable diseases in dairy cows, which can lead to enormous economic losses. Although many scholars have conducted a large number of studies on laminitis, the therapeutic test of medicinal plants in vitro is really rare. Licochalcone A is proved to possess anti-inflammatory and anti-oxidant properties. But the effect of licochalcone A on LPS-induced inflammatory claw dermal cells has not been discovered yet. In this study, the primary dairy cow claw dermal cells were treated with gradient concentrations of licochalcone A (1, 5, 10 µg/mL) in the presence of 10 µg/mL lipopolysaccharides (LPS). The results indicated that licochalcone A reduced the concentrations of inflammation mediators (TNF-α, IL-1β and IL-6), increased the activity of SOD, reduced the levels of MDA and ROS, downregulated the mRNA expressions of TLR4 and MyD88, suppressed the protein levels of p-IκBα and p-p65, and upregulated the protein expression of PPARγ. In summary, licochalcone A protected dairy cow claw dermal cells against LPS-induced inflammatory response and oxidative stress through the regulation of TLR4/MyD88/NF-κB and PPARγ signaling pathways.
Funder
Inovation ability training program of Hebei Province for Graduate candidate
Hebei key research and development program
Project of Bureau of Hebei Animal Husbandry and Veterinary Medicine
Hebei Dairy Cattle Innovation Team of Modern Agro-industry Technology Research System
Hebei Beef Innovation Team of Modern Agro-industry Technology Research System
Publisher
Springer Science and Business Media LLC
Cited by
3 articles.
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