Author:
Wang Jiabing,Ye Zhinan,Chen Yuan,Qiao Xinyu,Jin Yong
Abstract
AbstractSepsis is one of the most common causes of death in patients suffering from severe infection or injury. Currently, a specific effective therapy remains to be established. In the present study, miR-25-5p, miR-105, miR-106b-5p, miR-154-3p, miR-20b-5p, miR-295-3p, miR-291-3p, miR-301b, miR-352, and miR-93-5p were predicted to target TXNIP mRNA from the databases of miRDB, Targetscan, and microT-CDS. The luciferase reporter assay confirmed that miR-25-5p negatively regulates TXNIP expression. The ELISA analyses and western blotting demonstrated that miR-25-5p downregulated the production of IL-1β, IL-6, IL-8, and TNF-α in lipopolysaccharide (LPS)-stimulated cells or rats, as well as the protein levels of TXNIP, NLRP3, and cleaved caspase-1. In addition, miR-25-5p increased the cell viability and decreased the apoptosis in LPS-stimulated CTX TNA2 cells and reduced the abnormal morphology of the brain in LPS-stimulated rats. Besides, miR-25-5p decreased the relative mean fluorescence intensity of DCF in LPS-stimulated CTX TNA2 cell, apoptosis, and protein levels of MnSOD and catalase in LPS-stimulated brains. These findings indicate that miR-25-5p downregulated LPS-induced inflammatory responses, reactive oxygen species production, and brain damage, suggesting that miR-25-5p is a candidate treatment for septic encephalopathy.
Funder
Medical Health Science and Technology Project of Zhejiang Provincial Health Commission
Publisher
Springer Science and Business Media LLC
Reference28 articles.
1. Bone, R. C. et al. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine. Chest 101(6), 1644–1655 (1992).
2. Shapiro, L. & Gelfand, J. A. Cytokines and sepsis: Pathophysiology and therapy. New Horizons (Baltimore, Md). 1(1), 13–22 (1993).
3. Cossart, Y. E. The rise and fall of infectious diseases: Australian perspectives, 1914–2014. Med. J. Aust. 201(1 Suppl), S11–S14 (2014).
4. Singer, M. et al. The third international consensus definitions for sepsis and septic shock (sepsis-3). JAMA 315(8), 801–810 (2016).
5. Barbosa-Silva, M. C. et al. Infectious disease-associated encephalopathies. Crit. Care (Lond. Engl.). 25(1), 236 (2021).
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献