Author:
Maïza Auriane,Sidahmed-Adrar Nazha,Michel Patrick P.,Carpentier Gilles,Habert Damien,Dalle Carine,Redouane Walid,Hamza Magda,van Kuppevelt TH,Ouidja Mohand Ouidir,Courty José,Chantepie Sandrine,Papy-Garcia Dulce,Stettler Olivier
Abstract
Abstract
Heparan sulfate (HS) chains, covalently linked to heparan sulfate proteoglycans (HSPG), promote synaptic development and functions by connecting various synaptic adhesion proteins (AP). HS binding to AP could vary according to modifications of HS chains by different sulfotransferases. 3-O-sulfotransferases (Hs3sts) produce rare 3-O-sulfated HSs (3S-HSs), of poorly known functions in the nervous system. Here, we showed that a peptide known to block herpes simplex virus by interfering with 3S-HSs in vitro and in vivo (i.e. G2 peptide), specifically inhibited neural activity, reduced evoked glutamate release, and impaired synaptic assembly in hippocampal cell cultures. A role for 3S-HSs in promoting synaptic assembly and neural activity is consistent with the synaptic interactome of G2 peptide, and with the detection of Hs3sts and their products in synapses of cultured neurons and in synaptosomes prepared from developing brains. Our study suggests that 3S-HSs acting as receptors for herpesviruses might be important regulators of neuronal and synaptic development in vertebrates.
Funder
Vaincre Alzheimer’
H2020 European Institute of Innovation and Technology
Publisher
Springer Science and Business Media LLC
Cited by
10 articles.
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