Author:
Schultz Fabien,Anywar Godwin,Tang Huaqiao,Chassagne François,Lyles James T.,Garbe Leif-Alexander,Quave Cassandra L.
Abstract
AbstractAntibiotic resistance poses one of the greatest threats to global health today; conventional drug therapies are becoming increasingly inefficacious and limited. We identified 16 medicinal plant species used by traditional healers for the treatment of infectious and inflammatory diseases in the Greater Mpigi region of Uganda. Extracts were evaluated for their ability to inhibit growth of clinical isolates of multidrug-resistant ESKAPE pathogens. Extracts were also screened for quorum quenching activity against S. aureus, including direct protein output assessment (δ-toxin), and cytotoxicity against human keratinocytes (HaCaT). Putative matches of compounds were elucidated via LC–FTMS for the best-performing extracts. These were extracts of Zanthoxylum chalybeum (Staphylococcus aureus: MIC: 16 μg/mL; Enterococcus faecium: MIC: 32 μg/mL) and Harungana madagascariensis (S. aureus: MIC: 32 μg/mL; E. faecium: MIC: 32 μg/mL) stem bark. Extracts of Solanum aculeastrum root bark and Sesamum calycinum subsp. angustifolium leaves exhibited strong quorum sensing inhibition activity against all S. aureus accessory gene regulator (agr) alleles in absence of growth inhibition (IC50 values: 1–64 μg/mL). The study provided scientific evidence for the potential therapeutic efficacy of these medicinal plants in the Greater Mpigi region used for infections and wounds, with 13 out of 16 species tested being validated with in vitro studies.
Publisher
Springer Science and Business Media LLC
Reference100 articles.
1. Chandler, C. I. R. Current accounts of antimicrobial resistance: stabilisation, individualisation and antibiotics as infrastructure. Palgrave Commun. 5, 53. https://doi.org/10.1057/s41599-019-0263-4 (2019).
2. WHO. World health organization fact sheet: antibiotic resistance. https://www.who.int/news-room/fact-sheets/detail/antibiotic-resistance (2018).
3. O’Neill, J. Tackling Drug-Resistant Infections Globally: Final Report and Recommendations (Wellcome Trust and the UK Department of Health, London, 2016).
4. Thabit, A. K., Crandon, J. L. & Nicolau, D. P. Antimicrobial resistance: impact on clinical and economic outcomes and the need for new antimicrobials. Expert Opin. Pharmacother. 16, 159–177. https://doi.org/10.1517/14656566.2015.993381 (2015).
5. Rice, L. B. Progress and challenges in implementing the research on ESKAPE pathogens. Infect. Control Hosp. Epidemiol. 31(Suppl 1), S7-10. https://doi.org/10.1086/655995 (2010).
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