A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products
Author:
Funder
MEXT | Japan Society for the Promotion of Science
Ministry of Education, Culture, Sports, Science and Technology
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-019-42868-6.pdf
Reference40 articles.
1. Hui, E. K., Wang, P. C. & Lo, S. J. PCR-based strategies to clone unknown DNA regions from known foreign integrants. An overview. Methods Mol Biol 192, 249–274, https://doi.org/10.1385/1-59259-177-9:249 (2002).
2. Clark, J. M. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res 16, 9677–9686 (1988).
3. Lundberg, K. S. et al. High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene 108, 1–6 (1991).
4. Takagi, M. et al. Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR. Appl Environ Microbiol 63, 4504–4510 (1997).
5. Wang, Y. et al. A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro. Nucleic Acids Res 32, 1197–1207, https://doi.org/10.1093/nar/gkh271 (2004).
Cited by 8 articles. 订阅此论文施引文献 订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
1. Cloning and characterization of an acidic lipase from a lipolytic bacterium in tempeh;Journal of Genetic Engineering and Biotechnology;2023-12
2. A streamlined strategy for self-production of a commercial positive selection vector, the pJET1.2/blunt cloning vector, using common laboratory E. coli strains;3 Biotech;2023-06-06
3. A strategy for in-house production of a positive selection cloning vector from the commercial pJET1.2/blunt cloning vector at minimal cost;3 Biotech;2022-08-09
4. Recombinant jurkat cells (HMGN2-T cells) secrete cytokines and inhibit the growth of tumor cells;Journal of Molecular Histology;2022-07-21
5. Construction of a recombinant vector containing SAI gene using Gateway-based RNAi system: a preliminary study in the development of high sucrose sugarcane;IOP Conference Series: Earth and Environmental Science;2021-05-01
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3