Author:
von Auw Nadine,Serfling Robert,Kitte Reni,Hilger Nadja,Zhang Chengkang,Gebhardt Clara,Duenkel Anna,Franz Paul,Koehl Ulrike,Fricke Stephan,Tretbar U. Sandy
Abstract
AbstractProcess development for transferring lab-scale research workflows to automated manufacturing procedures is critical for chimeric antigen receptor (CAR)-T cell therapies. Therefore, the key factor for cell viability, expansion, modification, and functionality is the optimal combination of medium and T cell activator as well as their regulatory compliance for later manufacturing under Good Manufacturing Practice (GMP). In this study, we compared two protocols for CAR-mRNA-modified T cell generation using our current lab-scale process, analyzed all mentioned parameters, and evaluated the protocols’ potential for upscaling and process development of mRNA-based CAR-T cell therapies.
Funder
German Federal Ministry of Education and Research
Fraunhofer-Institut für Zelltherapie und Immunologie IZI
Publisher
Springer Science and Business Media LLC
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