Author:
Malá Zuzana,Žárská Ludmila,Malina Lukáš,Langová Kateřina,Večeřová Renata,Kolář Milan,Henke Petr,Mosinger Jiří,Kolářová Hana
Abstract
AbstractPhotodynamic inactivation (PDI) is a promising approach for the efficient killing of pathogenic microbes. In this study, the photodynamic effect of sulfonated polystyrene nanoparticles with encapsulated hydrophobic 5,10,15,20-tetraphenylporphyrin (TPP-NP) photosensitizers on Gram-positive (including multi-resistant) and Gram-negative bacterial strains was investigated. The cell viability was determined by the colony forming unit method. The results showed no dark cytotoxicity but high phototoxicity within the tested conditions. Gram-positive bacteria were more sensitive to TPP-NPs than Gram-negative bacteria. Atomic force microscopy was used to detect changes in the morphological properties of bacteria before and after the PDI treatment.
Publisher
Springer Science and Business Media LLC
Reference50 articles.
1. Farkas, J. Physical Methods of Food Preservation. In Food Microbiology: Fundamentals and Frontiers 3rd edn (eds Doyle, M. & Beuchat, L.) 685–712 (ASM Press, Washington, 2007).
2. Dobrynin, D., Fridman, G., Friedman, G. & Fridman, A. Physical and biological mechanisms of direct plasma interaction with living tissue. New J. Phys. 11, 115020 (2009).
3. Dillow, A. K., Dehghani, F., Hrkach, J. S., Foster, N. R. & Langer, R. Bacterial inactivation by using near- and supercritical carbon dioxide. PNAS 96(18), 10344–10348 (1999).
4. Ye, M. et al. A review of bacteriophage therapy for pathogenic bacteria inactivation inthe soil environment. Environ. Int. 129, 488–496 (2019).
5. Mohanty, S. et al. An investigation on the antibacterial, cytotoxic and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomed. Nanotechnol. 8, 916–924 (2012).
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