CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-018-26756-z.pdf
Reference42 articles.
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2. Eichinger, L. et al. The genome of the social amoeba Dictyostelium discoideum. Nature 435, 43–57, https://doi.org/10.1038/nature03481 (2005).
3. Gaudet, P., Pilcher, K. E., Fey, P. & Chisholm, R. L. Transformation of Dictyostelium discoideum with plasmid DNA. Nat Protoc 2, 1317–1324, https://doi.org/10.1038/nprot.2007.179 (2007).
4. Kuwayama, H. et al. PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. Nucleic acids research 30, E2 (2002).
5. Muramoto, T. et al. Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum. Mechanisms of development 120, 965–975 (2003).
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