A rapid influenza diagnostic test based on detection of viral neuraminidase activity

Author:

Lin Xuexiang,Liu Xiao-Yu,Zhang Bo,Qin Ai-Qing,Hui Kwok-Min,Shi Kevin,Liu Yang,Gabriel Don,Li X. James

Abstract

AbstractCurrent methods used for diagnosis of acute infection of pathogens rely on detection of nucleic acids, antigens, or certain classes of antibodies such as IgM. Here we report a virus enzyme assay as an alternative to these methods for detection of acute viral infection. In this method, we used a luciferin derivative as the substrate for detection of the enzyme activity of influenza viral neuraminidase as a means for diagnosis of influenza. The resulting commercial test, the qFLU Dx Test, uses a different supply chain that does not compete with those for the current tests. The assay reagents were formulated as a master mix that accommodated both the neuraminidase and luciferase reactions, thereby enabling rapid and prolonged production of stable light signal in the presence of influenza virus in the sample. The assay was evaluated using depository throat swab specimens. As expected, the assay exhibited similar detection rates for all influenza types and subtypes except for A(H7N9), which exhibited lower detection rate due to lower viral titer in the specimens. When throat swab specimens were diluted with the sample buffer of the test kit and tested with the qFLU Dx Test. The sensitivity and specificity were 82.41% (95% confidence interval: 79.66–85.84%) and 95.39% (95% confidence interval: 94.32–96.46%), respectively, for these diluted specimens in comparison to a real-time polymerase chain reaction assay. The uniqueness of the qFLU Dx Test as an enzymatic assay makes it highly complementary with currently available methods.

Funder

National Institutes of Health, United States

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Reference26 articles.

1. Heikkinen, T. Influenza in children. J. Acta Paediatr. 95(7), 778–784 (2010).

2. Centers for Disease Control and Prevention. Performance of rapid influenza diagnostic tests during two school outbreaks of 2009 pandemic influenza A (H1N1) virus infection—Connecticut. MMWR Morb. Mortal. Wkly. Rep. 58, 1029–1032 (2009).

3. Hui, K. M., Li, X. J., Pan, L., et al. A homogeneous biochemiluminescent assay for detection of influenza. In C. Spie Sensing Technology + Applications (2015).

4. Adamson, W. E. et al. Population exposure to a novel influenza A virus over three waves of infection. J. Clin. Virol. 52, 300–303 (2011).

5. Drexler, J. F. et al. Poor clinical sensitivity of rapid antigen test for influenza A pandemic (H1N1) 2009 virus. J. Emerg. Infect. Dis. 15, 1662–1664 (2009).

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3