Author:
Awadh Ammar A.,Le Gresley Adam,Forster-Wilkins Gary,Kelly Alison F.,Fielder Mark D.
Abstract
AbstractMycoplasmas are fastidious microorganisms, typically characterised by their restricted metabolism and minimalist genome. Although there is reported evidence that some mycoplasmas can develop biofilms little is known about any differences in metabolism in these organisms between the growth states. A systematic metabolomics approach may help clarify differences associated between planktonic and biofilm associated mycoplasmas. In the current study, the metabolomics of two different mycoplasmas of clinical importance (Mycoplasma pneumoniae and Mycoplasma fermentans) were examined using a novel approach involving nuclear magnetic resonance spectroscopy and principle component analysis. Characterisation of metabolic changes was facilitated through the generation of high-density metabolite data and diffusion-ordered spectroscopy that provided the size and structural information of the molecules under examination. This enabled the discrimination between biofilms and planktonic states for the metabolomic profiles of both organisms. This work identified clear biofilm/planktonic differences in metabolite composition for both clinical mycoplasmas and the outcomes serve to establish a baseline understanding of the changes in metabolism observed in these pathogens in their different growth states. This may offer insight into how these organisms are capable of exploiting and persisting in different niches and so facilitate their survival in the clinical setting.
Funder
Public Authority of Agriculture Affairs and Fish Resources (PAAF) in Kuwait
Publisher
Springer Science and Business Media LLC
Reference67 articles.
1. May, M., Whitcomb, R. F. & Brown, D. R. Mycoplasmaand related organisms. In Practical Handbook of Microbiology 2nd edn (eds Goldman, E. & Green, L. H.) 469–470 (CRC Press Taylor & Francis Group, New York, 2007).
2. Razin, S. The genus Mycoplasma and related genera (Class Mollicutes). In The Prokaryotes: A Hand Book on the Biology of Bacteria: Bacteria: Firmicutes, Cyanobacteria 3rd edn, Vol. 4 (eds Dworkin, M. et al.) 836–904 (University of Minnesota, Springer, Berlin, 2006).
3. Miles, R. J., Taylor, R. R. & Varsani, H. Oxygen uptake and H2O2 production by fermentative Mycoplasma spp. J. Med. Microbiol. 34, 219–223 (1991).
4. Rechnitzer, H., Rottem, S. & Herrmann, R. Reconstitution of an active Arginine deiminase pathway in Mycoplasma pneumoniae M129. Infect. Immun. 81, 3742–3749 (2013).
5. Taylor-Robinson, D. Infections due to species of Mycoplasma and Ureapalsma: an update. Clin. Inf. Dis. 23, 671–684 (1996).
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