Author:
Ito Akira,Yoshioka Kantaro,Masumoto Shinya,Sato Keiichiro,Hatae Yuki,Nakai Tomoki,Yamazaki Takashi,Takahashi Masazumi,Tanoue Shota,Horie Masanobu
Abstract
AbstractScale-up of production is needed for industrial applications and clinical translation of human induced pluripotent stem cells (hiPSCs). However, in cryopreservation of hiPSCs, successful rewarming of vitrified cells can only be achieved by convective warming of small volumes (generally 0.2 mL). Here, we present a scalable nano-warming technology for hiPSC cryopreservation employing inductive heating of magnetic nanoparticles under an alternating magnetic field. The conventional method by water bath heating at 37 °C resulted in a decrease of cell viability owing to devitrification caused by slow warming of samples with large volumes (≥ 20 mL). Nano-warming showed uniform and rapid rewarming of vitrified samples and improved viability of hiPSCs in the 20-mL system. In addition to single cells, hiPSC aggregates prepared using a bioreactor-based approach were successfully cryopreserved by the nano-warming technique. These results demonstrate that nano-warming is a promising methodology for cryopreservation in mass production of hiPSCs.
Funder
Japan Science and Technology Agency
Japan Society for the Promotion of Science
AJINOMOTO CO. INNOVATION ALLIANCE AMINO ACID RESEARCH PROGRAM
Ministry of Education, Culture, Sports, Science and Technology
Publisher
Springer Science and Business Media LLC
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