The impact of storage buffer, DNA extraction method, and polymerase on microbial analysis
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-018-24573-y.pdf
Reference40 articles.
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3. Brooks, J. P. et al. The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies. BMC Microbiol. 15, 66, https://doi.org/10.1186/s12866-015-0351-6 (2015).
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5. Ahn, J.-H., Kim, B.-Y., Song, J. & Weon, H.-Y. Effects of PCR cycle number and DNA polymerase type on the 16S rRNA gene pyrosequencing analysis of bacterial communities. J. Microbiol. 50, 1071–1074 (2012).
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