Author:
Puspitasari Anggraeini,Squarcio Fabio,Quartieri Martina,Totis Cristina,Hitrec Timna,Takahashi Akihisa,Yoshida Yukari,Hanamura Kenji,Yako Tomoko,Cerri Matteo,Simoniello Palma,Durante Marco,Tinganelli Walter
Abstract
AbstractHibernation or torpor is considered a possible tool to protect astronauts from the deleterious effects of space radiation that contains high-energy heavy ions. We induced synthetic torpor in rats by injecting adenosine 5′-monophosphate monohydrate (5′-AMP) i.p. and maintaining in low ambient temperature room (+ 16 °C) for 6 h immediately after total body irradiation (TBI) with accelerated carbon ions (C-ions). The 5′-AMP treatment in combination with low ambient temperature reduced skin temperature and increased survival following 8 Gy C-ion irradiation compared to saline-injected animals. Analysis of the histology of the brain, liver and lungs showed that 5′-AMP treatment following 2 Gy TBI reduced activated microglia, Iba1 positive cells in the brain, apoptotic cells in the liver, and damage to the lungs, suggesting that synthetic torpor spares tissues from energetic ion radiation. The application of 5′-AMP in combination with either hypoxia or low temperature environment for six hours following irradiation of rat retinal pigment epithelial cells delays DNA repair and suppresses the radiation-induced mitotic catastrophe compared to control cells. We conclude that synthetic torpor protects animals from cosmic ray-simulated radiation and the mechanism involves both hypothermia and hypoxia.
Funder
GSI Helmholtzzentrum für Schwerionenforschung GmbH
Publisher
Springer Science and Business Media LLC
Cited by
4 articles.
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