Author:
Kang Heekyoung,Sohn Hae-Jin,Seo Ga-Eun,Seong Gi-Sang,Ham A-Jeong,Park A-Young,Jung Suk-Yul,Lee Sang-Eun,Cho Shin-Hyeong,Shin Ho-Joon
Abstract
AbstractThe free-living amoebae Naegleria spp. and Acanthamoeba spp. exist in the natural environment and are sometimes causal agents of lethal primary amoebic meningoencephalitis (PAM), amoebic keratitis (AK) and granulomatous amebic encephalitis (GAE) in humans, respectively. To ascertain the existence of free-living amoebae in Korea, water samples were collected from the Korean hydrosphere, Namhangang (southern Han River), an active location for water skiing and recreation. Samples underwent two-step filtration and were cultured on non-nutrient agar medium with inactivated E. coli. The remaining samples were subjected to PCR for primarily the 18S small ribosomal RNA gene and gene sequencing. Similarities in 18S rDNA sequences, in comparison with various reference amoebae in GenBank, showed 86~99% homology with N. gruberi, N. philippinensis, N. clarki, A. polyphaga, A. castellannii, and Hartmannella (Vermamoeba) vermiformis. Therefore, this study will be useful for seasonal detection of free-living amoebae from various Korean hydrospheres in future studies.
Funder
National Research Foundation of Korea
Ministry of Health, Welfare and Family Affairs | Korea Centers for Disease Control & Prevention
Publisher
Springer Science and Business Media LLC
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