Author:
Fedele Chiara,Mäntylä Elina,Belardi Brian,Hamkins-Indik Tiama,Cavalli Silvia,Netti Paolo A.,Fletcher Daniel A.,Nymark Soile,Priimagi Arri,Ihalainen Teemu O.
Abstract
AbstractSurface topography is a key parameter in regulating the morphology and behavior of single cells. At multicellular level, coordinated cell displacements drive many biological events such as embryonic morphogenesis. However, the effect of surface topography on collective migration of epithelium has not been studied in detail. Mastering the connection between surface features and collective cellular behaviour is highly important for novel approaches in tissue engineering and repair. Herein, we used photopatterned microtopographies on azobenzene-containing materials and showed that smooth topographical cues with proper period and orientation can efficiently orchestrate cell alignment in growing epithelium. Furthermore, the experimental system allowed us to investigate how the orientation of the topographical features can alter the speed of wound closure in vitro. Our findings indicate that the extracellular microenvironment topography coordinates their focal adhesion distribution and alignment. These topographic cues are able to guide the collective migration of multicellular systems, even when cell–cell junctions are disrupted.
Funder
Emil Aaltosen Säätiö
Suomen Kulttuurirahasto
Academy of Finland
National Institutes of Health
Publisher
Springer Science and Business Media LLC
Cited by
29 articles.
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